Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: Identification of co-differentially expressed genes, including 6 upregulated and 13 downregulated genes, in the {"type":"entrez-geo","attrs":{"text":"GSE65409","term_id":"65409"}} GSE65409 and {"type":"entrez-geo","attrs":{"text":"GSE90062","term_id":"90062"}} GSE90062 datasets

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques:

Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: The expression levels of six overlapping DEGs in the normal and AML specimens from the GSE65409 and GSE90062 datasets. A, G. The expression levels of CDC20. B, H. The expression levels of CDC45. C, I. The expression levels of CDKN1A. D, J. The expression levels of CDKN2D. E, K. The expression levels of MCM2. F, L. The expression levels of MCM7.

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: Confirmation of the six hub DEGs in normal and AML specimens using gene expression profiling interactive analysis. A-F. Expressions of CDC20, CDC45, CDKN1A, CDKN2D, MCM2, and MCM7 were evaluated using The Cancer Genome Atlas database. G-L. Pearson’s correlation analysis of the relationships between these six hub genes. *P<0.05.

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: Clinicopathologic characteristics of patients with AML based on the protein expression levels of CDC45 and MCM7

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: Overexpression of CDC45 increased MCM7 expression, hindered AML cell proliferation, and blocked the G1/S phase transition. A. The protein expression levels of CDC45 and MCM7 in pDONR223-CDC45-transfected HL-60 and THP-1 cells. B. The protein expression levels of CDC45 and MCM7 in mEmerald-MCM7-transfected HL-60 and THP-1 cells. C. Representative images from the EdU assay using HL-60 and THP-1 cells after transfection with vector 1 and WT-CDC45. D. Representative images from the EdU assay using HL-60 and THP-1 cells after transfection with vector 2 and WT-MCM7. The ratio of EdU-positive cells was calculated as (EdU-positive cells/Hoechst-stained cells) ×100%. E. Representative images from the cell cycle assay using HL-60 and THP-1 cells after transfection with vector 1 and WT-CDC45. F. Representative images from the cell cycle assay using HL-60 and THP-1 cells after transfection with vector 2 and WT-MCM7. The percentages of cells in each cell cycle phase were expressed using a histogram. V1 and vector 1: pDONR223-vector; C and WT-CDC45: pDONR223-CDC45; V2 and vector 2: mEmerald-vector; M and WT-MCM7: mEmerald-MCM7; *P<0.05.

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques: Over Expression, Expressing, Sublimation, Transfection, EdU Assay, Plasmid Preparation, Staining, Cell Cycle Assay

Journal: American Journal of Translational Research

Article Title: CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia

doi:

Figure Lengend Snippet: Overexpression of CDC45/MCM7 suppressed the proliferation of AML cells by inhibiting the PI3K/AKT/mTOR signal pathway. Western blotting was performed to detect the protein expressions of PI3K, p-AKT, AKT, p-mTOR, mTOR, CDC45, and MCM7 in HL-60 cells (A) and THP-1 cells (C). Overexpression of either CDC45 or MCM7 in HL-60 and THP-1 cells changed the expression levels of PI3K, p-AKT, AKT, p-mTOR, and mTOR. Changes in the expression levels of these proteins were also examined in cells overexpressing CDC45 and treated with MCM7 siRNA. (B and D) Column graphs showing the results from the experiments shown in panels (A) and (C). vector 1: pDONR223-vector; WT-CDC45: pDONR223-CDC45; vector 2: mEmerald-vector; WT-MCM7: mEmerald-MCM7; CDC45+Con siRNA: pDONR223-CDC45 and control siRNA co-transfected; CDC45+MCM7: pDONR223-CDC45 and MCM7 siRNA co-transfected.

Article Snippet: The pDONR223_CDC45_WT_V5 plasmid was a gift from Jesse Boehm, Matthew Meyerson, and David Root (http://n2t.net/addgene:82990; RRID: Addgene_82990).

Techniques: Over Expression, Western Blot, Expressing, Plasmid Preparation, Transfection